Minimally invasive approach supported by the use of mouthguard in the treatment of sport-related root fracture: a case report.

All sporting activities have an associated risk of orofacial injuries that can affect the oral health-related quality of life (OHRQoL). A custom-made mouthguard can be used as an adjuvant treatment for faster recovery of an athlete to resume sporting activities. This case report described a minimally invasive approach by use of mouthguard for treating sport-related root fracture and its impact on OHRQoL. In a dental trauma care program, the patient was treated by repositioning the coronary portion and the confection of a splint. It was opted to not realize the endodontic treatment and the patient was inserted in a rigorous clinical and radiographic follow-up. A custom-made mouthguard was made, which allowed for the immediate return of athletes to practicing sports. The athlete's OHRQoL was negatively impacted by dental trauma, but the treatment proposed was associated with the use of custom-made mouthguards. After 2 years, the absence of radiographical lesions and a positive response to sensibility tests were observed. The success of the minimally invasive approach was attributed to immediate conduct following dental trauma, the implementation of follow-up, and associated with the use of custom-made mouthguard.

Differential amperometric determination of hydrogen peroxide in honeys using flow-injection analysis with enzymatic reactor.

Hydrogen peroxide (H2O2) present in honey was rapidly determined by the differential amperometric method in association with flow-injection analysis (FIA) and a tubular reactor containing immobilized enzymes. A gold electrode modified by electrochemical deposition of platinum was employed as working electrode. Hydrogen peroxide was quantified in 14 samples of Brazilian commercial honeys using amperometric differential measurements at +0.60V vs. Ag/AgCl((sat)). For the enzymatic consumption of H2O2, a tubular reactor containing immobilized peroxidase was constructed using an immobilization of enzymes on Amberlite IRA-743 resin. The linear dynamic range in H2O2 extends from 1 to 100 x 10(-6) mol L(-1), at pH 7.0. At flow rate of 2.0 mL min(-1) and injecting 150 microL sample volumes, the sampling frequency of the 90 determinations per hour is afforded. This method is based on three steps involving the flow-injection of: (1) the sample spiked with a standard solution, (2) the pure sample and (3) the enzymatically treated sample with peroxidase immobilized. The reproducibility of the current peaks for hydrogen peroxide in 10(-5) mol L(-1) range concentration showed a relative standard deviation (R.S.D.) better than 1%. The detection limit of this method is 2.9 x 10(-7) mol L(-1). The honey samples analyses were compared with the parallel spectrophotometric determination, and showed an excellent correlation between the methods.

Rapid determination of hydrogen peroxide using peroxidase immobilized on Amberlite IRA-743 and minerals in honey.

Hydrogen peroxide and trace metals (K+, Ca2+, Na+, Mg2+, Mn2+, and Li+) were determined in 14 samples of Brazilian commercial honeys. The method for the determination of H2O2 is based on selective oxidation of H2O2 using an on-line tubular reactor containing peroxidase immobilized on Amberlite IRA-743 resin. Reactors presented high stability for at least 2 weeks under intense use. The results show a simple, accurate, selective, and readily applied method to the determination of H2O2 in honey. The trace metals were determined by capillary zone electrophoresis. Mean contents of 656, 69.1, 71.8, 36.0, 21.4, and 1.70 mg/kg were found, respectively, for K+, Ca2+, Na+, Mg2+, Mn2+, and Li+ in the analyzed honeys. The cations were identified by comparison of the relative migration times of their peaks with the Ba2+ migration time used as reference. The electrophoretic analysis was simple and rapid and did not require any other preparation of sample than dilution and filtration.